Figure 7.

Benzyl alcohol sedation caused similar electrophysiological changes in slo^Δ55b and wild-type flies. A) Benzyl alcohol (BA) sedation increases the giant fiber following frequency of wild type (WT) and mutant (slo^Δ55b) animals to a similar degree. B) A stereotypical seizure response is shown. A constant low-frequency stimulus was applied throughout the duration of the recording to assess the responsiveness of the giant fiber pathway. An electroconvulsive stimulus (E) triggers a high-frequency initial discharge (ID), followed by a period of evoked response failures (Failure), and a delayed discharge (DD). After the delayed discharge the giant fiber has recovered (R) and responds normally to the low frequency stimulus. C) Benzyl alcohol (BA) sedation enhanced seizure susceptibility in both lines. Electroconvulsive stimuli of varying voltages ranging 5–50 V were utilized to determine seizure susceptibility, which is represented by the minimum stimulus voltage to trigger seizure. The average stimulus voltages in wild type and slo^Δ55b measured one day after benzyl alcohol (BA) sedation. The difference in seizure susceptibility in wild type and mutant animals does not differ. Unpaired Student t test, n = 5~9, *P ≤ 0.05. Error bars represent SEM.