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. Author manuscript; available in PMC: 2016 Jun 23.
Published in final edited form as: ACS Nano. 2015 May 14;9(6):6097–6109. doi: 10.1021/acsnano.5b01263

Figure 4.

Figure 4

Gene delivery agents G5 PAMAM, jetPEI , L-PEI, and DOTAP induce increased membrane currents. A) Representative traces for cells treated with the three polymers. Increased membrane current induced by G5 PAMAM (red) plateaus between 600 s – 740 s. Currents induced by jetPEI and L-PEI plateau at ~ 40 – 50 nA at higher concentrations. The first dotted line at 65 s indicates the release of the free cationic vectors resulting in exposure of these compounds to the cells. The second dotted line denotes the end of compound exposure. B) Average increase in current induced by the polymers. Cationic polymers jetPEI, LPEI and G5 PAMAM increase membrane permeability greater than DOTAP. C-E) Fluorescence images illustrating that cells exposed to the different gene delivery agents are intact after 900 s of exposure.