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Exposure of HEK 293 A cells to G5 PAMAM for 60 seconds induced increased membrane permeability that is not reversible for over 900 seconds. A) Representative current traces. The first dotted line indicates the release of G5 PAMAM at 65 s. Subsequent dotted lines indicate 10 s, 60 s and 600s from release of G5 PAMAM respectively when G5 PAMAM exposure was stopped and cells were exposed to ECS alone. B) The difference between currents at 65 s and subsequent time points.
