Figure 3.

Replication of LAC WT strain internalized into HeLa cells. HeLa cells were infected with either LAC WT or its isogenic mutant LAC∆psmα at a multiplicity of infection of 100:1 for 1 hour in DMEM medium. HeLa cells were then incubated for 1 hour in DMEM supplemented with gentamicin. At T2 and T6 after infection, cells were lysed, and CFUs were determined. LAC WT strain replicates intracellularly because the CFU number of the LAC WT strain was increased 6 hours after infection. *P < 0.05. The data are presented as the logarithm of CFU/10³ cells ± sd.