Figure 3.

Cell morphology and TRPC6 expression following I/R insult. Morphology of HK-2 cells (A) with 6-h ischemia (B) followed by 2-h (C), 6-h (D), or 12-h reoxygenation (E). Flow cytometric analysis of cell apoptosis and necrosis following I/R insult (F). Necrosis of the TRPC6 siRNA transfected cells was comparatively higher than that of NC siRNA-transfected or -non-transfected cells, while apoptosis was significantly different among the groups, which resulted in a significantly higher apoptosis/necrosis ratio in TRPC6 siRNA-transfected cells. * p<0.05 vs. the NC siRNA-transfected or -non-transfected groups, or vs. the corresponding group at the different reoxygenation times.