Table I.

Primer sequences for RT-qPCR.

Target	Primer sequences (5′→3′)	Annealing temperature (°C)	Cycle
NFATc1	F: 5′-TGC TCC TCC TCC TGC TGC TC-3′	58	32
	R: 5′-CGT CTT CCA CCT CCA CGT CG-3′
c-Fos	F: 5′-ATG GGC TCT CCT GTC AAC AC-3′	58	33
	R: 5′-GGC TGC CAA AAT AAA CTC CA-3′
RANK	F: 5′-AAA CCT TGG ACC AAC TGC AC-3′	53	32
	R: 5′-ACC ATC TTC TCC TCC CHA GT-3′
TRAP	F: 5′-ACT TCC CCA GCC CTT ACT ACC G-3′	58	30
	R: 5′-TCA GCA CAT AGC CCA CAC CG-3′
CTK	F: 5′-AGG CGG CTA TAT GAC CAC TG-3′	58	27
	R: 5′-CCG AGC CAA GAG AGC ATA TC-3′
CTR	F: 5′-TGC ATT CCC GGG ATA CAC AG-3′	59	40
	R: 5′-AGG AAC GCA GAC TTC ACT GG-3′
MMP-9	F: 5′-CGA CTT TTG TGG TCT TCC CC-3′	58	33
	R: 5′-TGA AGG TTT GGA ATC GAC CC-3′
CAII	F: 5′-CTC TCA GGA CAA TGC AGT GCT GA-3′	58	32
	R: 5′-ATC CAG GTC ACA CAT TCC AGC A-3′
GAPDH	F: 5′-ACT TTG TCA AGC TCA TTT CC-3′	58	30
	R: 5′-TGC AGC GAA CTT TAT TGA TG-3′

NFATc1, nuclear factor of activated T-cells cytoplasmic 1; RANK, receptor activator of nuclear factor-κB; TRAP, tartrate-resistant acid phosphatase; CTK, cathepsin K; CTR, calcitonin receptor; MMP-9, matrix metallopeptidase-9, CAII, carbonic anhydrase II.