Figure 5.

Bortezomib induces endoplasmic reticulum (ER) stress in vivo. Hepatic mRNA levels of (A) binding immunoglobulin protein (BiP) and (B) CCAAT-enhancer-binding protein homologous protein (CHOP) were measured using RT-qPCR at the indicated times after bortezomib (1 mg/kg) administration. (C) Elevated protein levels of inositol-requiring protein 1 (IRE-1)α, BiP, and CHOP by bortezomib administration are shown by western blot analysis (left panel), with results quantified in the right panel (n=6). Values are expressed as the means ± SEM (n=6). Data are representative of six independent experiments that provided similar results. ^*p<0.05, ^**p<0.01, ^***p<0.001.