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. 2016 Feb 3;37(3):631–638. doi: 10.3892/ijmm.2016.2478

Figure 4.

Figure 4

Effect of uncoupling protein 2 (UCP2) overexpression on high glucose (HG)-induced apoptosis and caspase activation in human umbilical vein endothelial cells (HUVECs). (A) Ad-UCP2 or adenovirus β-galactosidase (Ad-β-gal)-infected HUVECs treated with HG were double stained with Annexin V and PI using the Annexin V-FITC apoptosis detection kit. The proportion of apoptotic and necrotic cells was determined by FACSCalibur. (B) Effect of UCP2 overexpression on HG-induced apoptosis and necrosis. Apoptotic and necrotic cells were identified by staining with Annexin V (early apoptosis) or Annexin V/PI (late apoptosis) and PI, respectively. (C) Western blot analysis for the active subunit of caspase-3. (D) Caspase-3 activation was analyzed by measuring the activity using a caspase-3 colorimetric protease assay kit. The values are presented as the means ± SD of three independent experiments. *P<0.01 vs. control and **P<0.01 vs. β-gal.