Figure 6.

Effect of uncoupling protein 2 (UCP2) overexpression on reactive oxygen species (ROS) generation and NO production in human umbilical vein endothelial cells (HUVECs). Quiescent HUVECs were infected with Ad-UCP2 or Ad-β-gal. Two days after infection, cells were maintained in media containing 0.5% (v/v) FBS for 12 h, and exposed to 30 M high glucose (HG) for 6 h. (A) After 15 min incubation with 10 µmol/ml DCFH2-DA, the increase in DCFH2 oxidation was measured using FACSCalibur. (B) NO levels in HUVECs were measured in situ using DAF-FM diacetate. Values are presented as the means ± SD of three independent experiments. ^*P<0.01 vs. control and ^**P<0.01 vs. β-gal.