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Exposure to high glucose inhibits the interaction between bradykinin receptor B2 (BK2R) and EphB4/EphrinB2. Osteoblasts were incubated with high glucose (16.5 mM) for 4 days. Lysates from (A) osteoblasts and (B) tibias of mice were immunoprecipitated with anti-BK2R antibody. Immunopellets were analyzed by western blot analysis (immunoblotting, IB) using anti-EphB4 or anti-EphrinB2 antibody. IP, immunoprecipitation.
