Table 2. Target organisms, target genes, optimized annealing temperatures (T_a), and primer concentrations used in real-time quantitative PCR.

Target organism	Target gene	Ta (°C)	Primer conc. (nM)	Reference
Protozoa	18S rRNA	55	600	[30]
Methanogens	mcrA	60	900	[31]
Domain bacteria	16S rRNA	50	500	[32]
Fibrobacter succinogenes	16S rRNA	58	200	[32]
Ruminococcus albus	16S rRNA	55	500	[33]
Ruminobacter amylophilus	16S rRNA	60	500	[32]
Prevotella bryantii	16S rRNA	61	500	[32]
Selenomonas ruminantium	16S rRNA	59	500	[32]
Clostridium aminophilum	16S rRNA	56	400	[14, 34]