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. 2016 Feb 29;12(2):e1005885. doi: 10.1371/journal.pgen.1005885

Fig 5. Specific activity and ATP Km of EphA3 WT and extended R-spine network mutants.

Fig 5

The single extended R-spine mutants exhibit loss of specific activity and an increase in ATP Km that can be partially rescued by addition of STK-histidine. Extended R-spine double mutants show partial restoration of specific activity with the addition of STK-histidine, but not to WT levels. Partial rescue of ATP Km is also seen for double mutants. No activity was observed for the kinase dead control (K653M) or for the R-spine-Asp mutant (D806N). For each mutant, ATP Km was measured at 12 ATP concentrations and replicated at least 5 times for each mutant. The histogram and error bars represent the average and standard deviation of values from replicate experiments.