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. 2016 Feb 29;11(2):e0150400. doi: 10.1371/journal.pone.0150400

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© 2016 Abadjieva, Kistanova

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — mRNA level of GDF9 and BMP15 in the oocytes (A) and cumulus cells (B) of the female rabbits in dependence on age. The results were obtained by comparison of the control groups from both generations–does-mothers and F1 female offspring. Total RNA was extracted from the oocytes and cumulus cells and subjected to real-time PCR to determine the mRNA levels of BMP15 and GDF9. The expression of these mRNAs was normalized to the expression of control gene GAPDH. The triplicates for each reaction were averaged. This data represent the mean ±SEM of the combined results from the analysis of seven rabbits from each group (*, P < 0.05). The significantly higher expression of BMP15 and GDF9 in the oocytes from the ovaries of F1 female compared to the mothers' generation was observed.