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. Author manuscript; available in PMC: 2016 Nov 5.
Published in final edited form as: Mol Cell. 2015 Oct 17;60(3):374–384. doi: 10.1016/j.molcel.2015.09.009

Figure 3. ATPase assays with aPLs for RecA and DNA Gyrase.

Figure 3

(A) Anionic PLs inhibit DNA dependent ATP hydrolysis by RecA. (B) Negative control with DNA gyrase DNA-dependent ATP hydrolysis indicates that the inhibition of RecA ATP hydrolysis is due to a specific interaction of anionic PLs with RecA. (C) Titration of PG liposomes show that ATPase rates are inhibited as a function of concentration of PG. (D) Absolute ATPase rates calculated from fits to the curves above. Error bars indicate SD. Also see Fig S3.