TABLE 8.
Geometric mean limits of detection in pure cultures according to culture method and β-lactamase classa
| Method | Geometric mean limit of detection (CFU/ml) |
No. of isolates of class A/B/D | Reference | ||
|---|---|---|---|---|---|
| Class A | Class B | Class D | |||
| Supercarba | 1.47 × 101 | 3.36 × 101 | 1.37 × 101 | 18/52/44 | 149 |
| chromID ESBL | 1 × 101 | 1.26 × 101 | 1.21 × 103 | 18/52/44 | |
| chromID KPC | 8.1 × 101 | 1.64 × 103 | 3.43 × 106 | 18/52/44 | |
| Supercarba | 1.41 × 101 | 2.81 × 101 | 1.59 × 101 | 20/51/43 | 143 |
| Brilliance CRE | 1.12 × 102 | 2.86 × 102 | 1.45 × 103 | 20/51/43 | |
| CHROMagar KPC | 5.89 × 101 | 8.93 × 102 | 4.62 × 106 | 20/51/43 | |
| MacConkey agar with imipenem | 4.68 × 102 | 1.24 × 103 | NT | 8/2/0 | 80 |
| MacConkey agar with meropenem/ertapenem disks | 2.62 × 106 | 3.32 × 105 | NT | 8/2/0 | |
| CHROMagar KPC | 2.02 × 103 | 1.24 × 104 | NT | 8/2/0 | |
| CDC method | 6.87 × 101 | 8.66 × 102 | 5.2 × 107 | 5/2/1 | 140 |
| chromID ESBL with prior enrichment in BHI broth + 10 μg ertapenem | 2.6 × 101 | 5.55 × 101 | ND | 5/2/1 | |
| chromID ESBL | 7.49 × 101 | 4.42 × 102 | ND | 5/2/1 | |
| chromID Carba | 2.11 × 101 | 4.42 × 102 | 5.5 × 107 | 5/2/1 | |
| Brilliance CRE | 2.67 × 101 | 3.41 × 101 | 3.77 × 101 | 12/14/5 | 166 |
| chromID OXA-48 | 1 × 107 | ND | 3.36 × 101 | 10/10/57 | 167 |
| chromID Carba | 1 × 101 | 2.0 × 101 | 1.62 × 104 | 10/10/57 | |
| Supercarba | 3.16 × 101 | 2.51 × 102 | 2.98 × 101 | 10/10/57 | |
| CHROMagar KPC | ND | ND | 1.26 × 104 | 0/0/9 | 100 |
| CHROMagar ESBL | ND | ND | 5.26 × 103 | 0/0/9 | |
a
NT, not tested; ND, not detected; BHI, brain heart infusion.