TABLE 1.

Primers and probe used for reverse transcription and quantitative PCR

Purpose and primer or probe	Sequencea	Genome region (positions)	Fragment size (no. of bases)a	Reference
Reverse transcription
Reverse primer 3424	TCT TTC GAG CAC ACC CAC C	3405–3424	2,169	24
Reverse primer 2440	TCT ATA CCA ACG GAT TTG AGC C	2418–2440	1,185	This work
Quantitative PCR
Forward primer	TCG ATG GTC CAT ACC TTA GAT GC	1255–1277		26
Reverse primer	ACC CCG TTA GCG AAG TTG CT	1404–1423		26
Probe	5′–FAM–TC TCG TCG ACA ATG GC–3′–BHQ-2b	1362–1375		26

^aSize of the RNA analyzed using this reverse primer for reverse transcription and in reference to the site used for the real-time PCR assay.

^bFAM, 6-carboxyfluorescein; BHQ-2, black hole quencher 2 dye.