Histochemical staining and level of reactive oxygen species in Atractylodes lancea inoculated with Pseudomonas fluorescens ALEB7B. (A) Histochemical staining of hydrogen peroxide (H2O2) and superoxide anions in plant leaves and roots after 10 days. (B to E) Level of H2O2, singlet oxygen, superoxide anions, and hydroxyl radicals in A. lancea over time. Results are means from three biological replicates. Error bars indicate standard deviations. *, significant differences at P < 0.05; **, significant differences at P < 0.01. CK, control; BAC, P. fluorescens ALEB7B.