FIG 1.

Phylogenetic trees based on 24 backbone gene proteins (a) and TrfA (b) showing the relationship of the plasmids pBHB, pTB30, pCNB1, and pI2 to five IncP-1 subgroup plasmids. The ML/MP/NJ bootstrap values are sequentially indicated at the branches, and the asterisks indicate that all three values are 100. The α, β, β-2, δ, and ε taxonomies of IncP-1 subgroup plasmids are marked by vertical lines with different colors. The 24 backbone gene protein-based and TrfA-based phylogenetic trees are in accordance, except for a slight discrepancy in the plasmid pI2. The plasmids from the genus Comamonas are in boldface italics. R, resistance plasmid; C, catabolic plasmid. The substrates of catabolic plasmids are as follows: pEST4011, 2,4-dichlorophenoxyacetic acid; pI2, 3-chloroaniline; pA81, chlorobenzoate; pWDL7::rfp, 3-chloroaniline; pCNB1, 4-chloronitrobenzene; pTB30, 3-chloroaniline; pTSA, p-toluenesulfonate; pBHB, 3,5-dibromo-4-hydroxybenzonitrile; pJP4, 2,4-dichlorophenoxyacetic acid.