TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| Comamonas sp. strain 7D-2 | Bromoxynil-degrading strain with plasmid pBHB; bxn2+ bhb+ Gmr | 14 |
| Comamonas sp. strain 2B | Plasmid pBHB-cured mutant of Comamonas sp. strain 7D-2; bxn2− bhb− Gmr | 14 |
| Comamonas sp. strain 2B-bhbAB | Complementary strain of Comamonas sp. strain 2B containing pMCSQ2-bhbAB | 14 |
| Comamonas sp. strain 2B-bhbA2B2 | Complementary strain of Comamonas sp. strain 2B containing pMCSQ2-bhbA2B2 | This study |
| Comamonas sp. strain 2B-bhbA2B | Complementary strain of Comamonas sp. strain 2B containing pMCSQ2-bhbA2B | This study |
| Comamonas sp. strain 2B-bhbAB2 | Complementary strain of Comamonas sp. strain 2B containing pMCSQ2-bhbAB2 | This study |
| E. coli DH5α | Host strain for cloning vectors | TaKaRa |
| E. coli HB101(pRK2013) | Conjugation helper strain; Kmr | Laboratory stores |
| Plasmids | ||
| pBBR1MCS-2 | Broad-host-range vector | 44 |
| pMCSQ2 | 527-bp fragment containing promoter sequence upstream of bhbF inserted into the HindIII-BamHI sites of pBBR1MCS2 | 14 |
| pMCSQ2-bhbAB | 4,293-bp fragment containing bhbAB inserted into the BamHI-XbaI sites of pMCSQ2 | 14 |
| pMCSQ2-bhbA2B2 | 4,252-bp fragment containing bhbA2B2 inserted into the BamHI-XbaI sites of pMCSQ2 | This study |
| pMCSQ2-bhbB | 1,046-bp fragment of bhbB inserted into the HindIII-XbaI sites of pMCSQ2 | This study |
| pMCSQ2-bhbB2 | 1,023-bp fragment of bhbB2 inserted into the HindIII-XbaI sites of pMCSQ2 | This study |
| pMCSQ2-bhbA2B | 323-bp fragment of bhbA2 with BamHI-XbaI sites inserted into the BglII-XbaI sites of pMCSQ2-bhbB | This study |
| pMCSQ2-bhbAB2 | 3,254-p fragment of bhbA with BamHI-XbaI sites inserted into the BglII-XbaI sites of pMCSQ2-bhbB2 | This study |