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. 2016 Feb 19;82(5):1549–1555. doi: 10.1128/AEM.03615-15

FIG 1.

FIG 1

Verification of knockout of mxaF in M. trichosporium OB3b by PCR. (A) PCR of mxaF. Lane 1, PCR of mxaF from M. trichosporium OB3b mxaF::Gmr; lane 2, PCR of mxaF from M. trichosporium OB3b wild type; lane 3, PCR of mxaF from plasmid construct pMFU01 (positive control); lane 4, PCR of mxaF from nuclease-free water (negative control); lane M, molecular size markers. (B) PCR of the pk18mobsacB backbone. Lane 1, PCR of the pk18mobsacB backbone in M. trichosporium OB3b mxaF::Gmr; lane 2, PCR of the pk18mobsacB backbone in the M. trichosporium OB3b wild type; lane 3, PCR of the pk18mobsacB backbone in pMFU01 (positive control); lane 4, PCR of the pk18mobsacB backbone in nuclease-free water (negative control); lane M, molecular size markers.