TABLE 1.
Primers used in this study
| Primer | Targeted gene | Sequence (5′–3′)a | Reference or source |
|---|---|---|---|
| mxaF_OB3b_F3 | mxaF (arm A) | GCTGCTGAATTCCGTATCCC | This study |
| mxaF_OB3b_R3 | ATTTTTGGATCCACGTCATAGGCGGTCATGTA | This study | |
| mxaF_OB3b_F4 | mxaF (arm B) | ATTTTTGGATCCATCATTTGGCCAAGGACGTG | This study |
| mxaF_OB3b_R4 | ATTTTTAAGCTTAGGTTCACGTCGTCATAGGG | This study | |
| qpmoA_FO | pmoA | TTCTGGGGCTGGACCTAYTTC | 42 |
| qpmoA_RO | CCGACAGCAGCAGGATGATG | ||
| qmmoX_FO | mmoX | TCAACACCGATCTSAACAACG | 42 |
| qmmoX_RO | TCCAGATTCCRCCCCAATCC | ||
| qxoxF1_FO | xoxF1 | TCAAGGACAAGGTGTTCGTC | 20 |
| qxoxF1_RO | CGAGCCGTCCTTGATGTTAT | ||
| qxoxF2_FO | xoxF2 | GCGCGAAGGATTGGGAATAT | 20 |
| qxoxF2_RO | GCCTCGTAATTCATGCACAG |
a
Underlined sequences represent different restriction sites. Y, S, and R, the IUPAC DNA codes for the C/T, C/G, and A/G nucleobases, respectively.