FIG 5.

Effect of the class I RNR genes or AdoCbl supplementation on growth. (A) Growth of the WT (circles), PFW (squares), and PFW I-RNR (triangles) strains in TR medium or synthetic medium with (filled symbols) or without (open symbols) supplementation with 0.1 μg/ml AdoCbl. A preculture with an OD₆₀₀ of around 1.1 was diluted 1:100 into fresh medium and incubated with shaking at 70°C. (B) Count of viable cells at the same OD₆₀₀ of culture. Each strain was cultured in TR medium with (solid bars) or without (hatched bars) AdoCbl supplementation and was spread on a TR plate when the OD₆₀₀ reached 0.15.