TABLE 1.
PCR primer sequences to amplify ca. 5-kbp regions for homologous recombination with pTT27
| Locus | Location on pTT27 (nt) | Length of region (bp) | Primer sequencea |
|---|---|---|---|
| 1 | 231441–3800 | 4,965 | 5′-AAGCGGCCGCGAATTCCTACCTGCCCCAGGACCTTCCCGATGGAAAGCGGCAGGAG |
| 5′-AAGCGGCCGCGTCGACTTGAGCTTGTGGCCATCGCCGGGGTCTACCTCGAGGGGTACGAGG | |||
| 2 | 59961–65394 | 5,434 | 5′-AAGCGGCCGCGAATTCGCAGATCTTCTCCTTTGAGCCCCTCCTCATCGGAGGGAAC |
| 5′-AAGCGGCCGCGTCGACGCCTTGGCCCACTTCACCGGCTTCAAGGCCACTTCCGACC | |||
| 3 | 119941–125680 | 5,740 | 5′-AAGCGGCCGCGAATTCGAGGCAACTTGCTGAGGTCCAGGACGTTCTGCCGGGAACC |
| 5′-AAGCGGCCGCGTCGACCCGCTTCATGGGTACCTCCAGCGCACCTCGGGGTCGGACATG | |||
| 4 | 179931–185270 | 5,340 | 5′-AAGCGGCCGCGAATTCTTCACGCCTCCGGTTTGACGACCGGGTACATGTATCTTGC |
| 5′-AAGCGGCCGCGTCGACTAAGGACGGCGACGATGTTGAGGTTCTCCGTGTAGTTCGG |
a
The underlined, boldface, and italic sequences indicate NotI, EcoRI, and SalI sites, respectively.