TABLE 1.
Pseudomonas protegens Pf-5 and derivative strains used in this study
| Strain | Designation | Descriptiona | Reference(s) |
|---|---|---|---|
| Pf-5 | JL4585 | Soil isolate; produces Ofa, Prn, Plt, HCN, MAPG, DAPG, Rzx | 3, 68 |
| Mutants of Pf-5 | |||
| ΔgacA | JL4577 | Insertion of aphI at the site of a 626-bp (nt 1–626) deletion in gacA; altered in the many phenotypes regulated by GacA; Kmr | 16 |
| ΔofaA | JL4807 | 1,143-bp deletion internal to ofaA; has FRT scar; Ofa− | 16 |
| ΔphlA | LK023 | 639-bp deletion of BglII fragment internal to phlA; DAPG−, MAPG− | 9 |
| ΔphlD | JL4804 | 162-bp deletion in phlD; has FRT scar; DAPG−, Plt− | 49, 9 |
| ΔpltA | JL4805 | 275-bp deletion in pltA; has FRT scar; Plt− | 50 |
| ΔprnC | JL4793 | 86-bp insertion of FRT site into prnC; Prn− | 50 |
| ΔrzxB | JL4808 | 1,342-bp deletion in rzxB; has FRT scar; Rzx− | 50 |
| ΔhcnB | JL4809 | 239-bp deletion in hcnB; has FRT scar; HCN− | 53 |
| ΔphlD ΔprnC | JL4830 | DAPG−, MAPG−, Plt−, Prn−; derived by adding a phlD deletion to JL4793 | This study |
| ΔphlA ΔprnC | LK026 | DAPG−, MAPG−, Prn− | 50 |
| ΔphlA ΔrzxB | LK027 | DAPG−, MAPG−, Rzx− | 50 |
| ΔphlD ΔrzxB | JL4901 | DAPG−, MAPG−, Plt−, Rzx−, derived by adding an rzxB deletion to JL4804 | This study |
| ΔprnC ΔrzxB | JL4902 | Prn−, Rzx−; derived by adding an rzxB deletion to JL4793 | This study |
| ΔphlD ΔrzxB ΔprnC | JL4844 | Prn−, DAPG−, MAPG−, Plt−, Rzx−; derived by adding an rzxB deletion to JL4830 | This study |
| ΔphlA ΔrzxB ΔprnC | LK031 | Prn−, DAPG−, MAPG−, Rzx− | 50 |
| ΔphlD ΔrzxB ΔprnC ΔpltA | JL4855 | Prn−, DAPG−, MAPG−, Plt−, Rzx−; derived by adding a pltA deletion to JL4844 | This study |
| ΔphlD ΔrzxB ΔprnC ΔhcnB ΔpltA | JL4865 | Prn−, DAPG−, MAPG−, Plt−, Rzx−, HCN−; derived by adding an hcnB deletion to JL4855 | This study |
| ΔphlD ΔrzxB ΔprnC ΔhcnB ΔpltA ΔofaA | JL4909 | Prn−, DAPG−, MAPG−, Plt−, Rzx−, HCN−, Ofa−; derived by adding an ofaA deletion to JL4865 | This study |
| ΔphlD ΔrzxB ΔprnC ΔhcnB ΔpltA ΔofaA ΔtoxB | JL4932 | Prn−, DAPG−, MAPG−, Plt−, Rzx−, HCN−, Ofa−, Tox−; derived by adding a toxB deletion to JL4909 | This study |
a
Phenotype abbreviations: DAPG, 2,4-diacetylphloroglucinol; HCN, hydrogen cyanide; MAPG, monoacetylphloroglucinol; Ofa, orfamide A; Plt, pyoluteorin; Prn, pyrrolnitrin; Rzx, rhizoxin derivatives; Tox, toxoflavin. Mutants of Pf-5 containing deletions in ofaA, phlD, pltA, prnC, rzxB, hcnB, and toxB have FRT scars (85- to 86-bp Flp recombinase target sites) in those genes. In-frame deletions were generated in phlA, and the deleted gene does not have an inserted FRT sequence. Primers used to generate and confirm each mutation are provided in the reference to the corresponding single mutant or in Table 2. nt, nucleotides.