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. 2016 Feb 19;82(5):1372–1382. doi: 10.1128/AEM.02574-15

TABLE 3.

Concentrations of antibiotics produced in culture by P. protegens Pf-5 and derivative strains having mutations in biosynthetic genesa

Expt and strain/genotype Designation Concn, μg ml−1 (mean ± SEM)b
2,4-Diacetylphloroglucinol Monoacetylphloroglucinol Pyoluteorin Pyrrolnitrin Rhizoxin WF-1360F Orfamide A
1
    Pf-5 JL4585 0.10 ± 0.04 0.13 ± 0.06 8.0 ± 2.1 1.2 ± 0.3 4.4 ± 0.4 100 ± 14
    ΔprnC JL4793 0.47c 0.73c 7.6 ± 0.4 BD 5.6 ± 0.7 110 ± 14
    ΔpltA JL4805 7.2 ± 0.4 5.6 ± 0.6 BD 1.0 ± 0.1 8.1 ± 0.4 150 ± 3
    ΔrzxB JL4808 0.04 ± 0.01 0.07 ± 0.02 8.0 ± 0.7 1.1 ± 0.1 BD 100 ± 7
    ΔhcnB JL4809 9.5 ± 0.9 12 ± 1 11 ± 7 1.6 ± 0.3 36 ± 11 270 ± 20
    ΔphlA LK023 BD BD 7.2 ± 1.8 1.2 ± 0.2 3.8 ± 0.5 99 ± 11
2
    Pf-5 JL4585 0.26 ± 0.15 0.65 ± 0.36 8.0 ± 0.5 1.5 ± 0.1 4.5 ± 0.1 12 ± 1
    ΔofaA JL4807 0.03 ± 0.01 0.09 ± 0.03 7.5 ± 0.6 1.6 ± 0.3 2.4 ± 0.1 0.73 ± 0.01
    ΔphlA ΔrzxB ΔprnC LK031 BD BD 8.0 ± 0.1 BD BD 110 ± 2
3
    Pf-5 JL4585 0.05 ± 0.02 0.14 ± 0.1 5.9 ± 0.4 1.3 ± 0.1 3.8 ± 0.1 92 ± 1
    ΔgacA JL4577 BD BD BD BD BD BD
    ΔphlD JL4804 BD BD BD 0.63 ± 0.04 4.0 ± 0.2 130 ± 7
    ΔphlD ΔrzxB ΔprnC ΔhcnB ΔpltA ΔofaA ΔtoxB JL4932 BD BD BD BD BD 0.54 ± 0.11
4
    Pf-5 JL4585 1.4 ± 0.4 2.4 ± 0.6 5.5 ± 0.6 1.1 ± 0.1 5.0 ± 0.1 110 ± 7
    ΔrzxB ΔprnC JL4902 0.30 ± 0.21 0.9 ± 0.4 6.2 ± 0.6 BD BD 90 ± 1
    ΔphlA ΔrzxB LK027 BD BD 5.8 ± 0.1 1.2 ± 0.1 BD 88 ± 4
5
    Wild type JL4585 0.16 ± 0.04 0.33 ± 0.07 4.0 ± 2.9 0.70 ± 0.51 2.3 ± 1.4 56 ± 32
    ΔphlD ΔprnC JL4830 BD BD BD BD 7.9 ± 0.8 130 ± 14
    ΔphlD ΔrzxB ΔprnC JL4844 BD BD BD BD BD 130 ± 7
    ΔphlD ΔrzxB ΔprnC ΔhcnB ΔpltA JL4865 BD BD BD BD BD 300 ± 1
    ΔphlD ΔrzxB JL4901 BD BD BD 1.0 ± 0.1 BD 120 ± 1
a

P. protegens Pf-5 or derivative strains were grown in 5 ml of NYBGly supplemented with 0.35 mM zinc sulfate (NYBGly-Zn). Cultures were incubated for 48 h at 20°C with shaking at 200 rpm before the concentrations of the antibiotics were quantified by HPLC.

b

The values represent the mean and standard error of the mean for two replicates, each composed of four broth cultures. Values were rounded to two significant digits. BD, below detection, with detection limits being approximately 0.02 μg ml−1 for each compound.

c

The compound was detected in only one of the two replicates. The value shown is one half the concentration determined from the replicate in which the compound was detected.