FIG 3.

Cytosolic translocation of F. noatunensis subsp. noatunensis is dependent on IglC. Ultrastructural analysis of high-pressure-frozen Dictyostelium wt cells infected with F. noatunensis subsp. noatunensis (F.n.n.) wt (A to D) and ΔiglC (E to G) was performed. Shown are representative examples of overviews (A and E) and high-magnification images at 1 (B and F), 4 (C and G), and 24 (D) hpi. *, intact F. noatunensis subsp. noatunensis; ×, digested F. noatunensis subsp. noatunensis; arrows, membranes enclosing bacteria; arrowheads, electron-pale areas characteristic of F. noatunensis subsp. noatunensis after HPF. Scale bars, 2 μm (A and E) and 500 nm (B to D, F, and G).