TABLE 1.
Diagnostic PCR to determine presence of Pvrbp genes
| Gene | Primer sequencea | Primer positionb (base) | Product size (bp) |
|---|---|---|---|
| Pvrbp1a | ACACGATGCAGGTGCAGA | 8264 | 538 |
| ACCCATACGTATACCTACCAG | 8801 | ||
| Pvrbp1b | CGGATGAAGCTGACAAGGA | 4642 | 853 |
| GGGTCCGAATTCTGGGATA | 5494 | ||
| Pvrbp2a | GGCAAGGCAAATTGTTCAGC | 7321 | 122 |
| ACGGACATTTCCATTTTCATTTCT | 7442 | ||
| Pvrbp2b | CACAACATGAGGATCAGTCACAAC | 8468 | 94 |
| ATACCTGAAAAGACAGATAATCCCAAA | 8561 | ||
| Pvrbp2c | GTGGGAAACTTTTCTTCCCTCT | 5431 | 166 |
| CTGAAGTGTTTTCCGAAGCA | 5596 | ||
| Pvrbp3 | CTGGTGACAACCATTCTGATGAC | 8413 | 187 |
| AAATCGGCTTCTTGTGATTCATG | 8599 | ||
| Pvrbp2e | CGACTGCTACAAACATATTAGATAACACA | 4336 | 111 |
| TCTGAGCTACCGCTTCTTCTAATAATT | 4466 | ||
| CGGAATTGCAAGACAAAGC | 2299 | 844 | |
| GCGATCGATTGTTCGTTTG | 3142 | ||
| TCGACAAAGGCATGGAAA | 3367 | 1,314 | |
| CGCTGTGCTTAACCGTTTT | 4680 | ||
| GCCGAATTAGACAAACATTCA | 6333 | 1,494 | |
| TTTACGCGTTCCTTTTCACC | 7826 | ||
| Pvrbp2p1 | TTGTTTCTACTTGTGAAGAAGCCAAA | 1622 | 126 |
| CATCATAACTYTGTTTAACTGATGCAATT | 1747 | ||
| Pvrbp2d | CAGAAGAGAAACTTTAGAAAGTGACCT | 7402 | 86 |
| CTTCACTTAATGTATTCGCATTTAATT | 7487 |
a
Primer sequences are shown from 5′ to 3′. Primer pairs are listed in the same order as in Fig. 2.
b
The position of the 5′ end of each primer is relative to the start codon of each gene in the Sal-1 reference strain, except for Pvrbp2e, which is absent from the strain. The positions of Pvrbp2e primers are based on the sequence of the VTTY57 isolate in this study.