FIG 1.

Expression of glycolytic enzymes of M. pneumoniae in E. coli and reactivity of guinea pig antisera to recombinant proteins. (A) The expression of recombinant proteins was analyzed by SDS-PAGE and Coomassie blue staining. Arrow, expected molecular mass of glycolytic enzyme. (B) Reaction of guinea pig antisera to recombinant proteins against total proteins of M. pneumoniae in immunoblotting. PIS, preimmune serum. (C) Reactivity of antisera with total proteins of M. pneumoniae in ELISA. The experiment was repeated twice, with eight replicates each time. The results from one experiment are shown, and bars represent means and standard deviations.