FIG 3.

Localization of glycolytic enzymes on the surfaces of M. pneumoniae cells. (A) Examples of results. Colony blot using antiserum to whole proteins of M. pneumoniae and to recombinant proteins (scale bar, 200 μm). Chymotrypsin treatment of M. pneumoniae cells and incubation of blotted total proteins with antiserum to recombinant proteins were performed (lane 1, 0 μg; lane 2, 5 μg; lane 3, 10 μg; and lane 4, 40 μg of chymotrypsin). Immunofluorescence of fixed M. pneumoniae cells after incubation with antisera to TX100-insoluble proteins (control) and to recombinant proteins (scale bars, 10 μm), respectively, was assessed. –, Negative reaction; +, positive reaction. (B) Summary of results.