FIG 5.

Binding of human Plg to surface-displayed glycolytic enzymes of M. pneumoniae analyzed by ELISA. (A) Influence of preincubation of Plg with corresponding guinea pig antisera (1:50) and preimmune serum (PIS) on binding to immobilized recombinant proteins. ***, P < 0.001. (B) Binding of Plg to recombinant proteins in the presence and absence of 650 mM NaCl. **, P < 0.01; *, P < 0.05. (C) Binding of Plg in the presence and absence of 1 mM ε-aminocaproic acid (ACA). Recombinant protein PdhB-P3 was used as control for a Plg-binding protein without ACA influence on this interaction (25, 26). All experiments were carried out twice, with eight wells each. The means and standard deviations of one experiment are shown.