TABLE 1.
Characteristics of glycolytic enzymes of M. pneumoniae
| Enzyme (abbreviation) | Genea | No. of TGA codon(s) | Putative mol mass (kDa) | Phosphorylationb | Remarksc |
|---|---|---|---|---|---|
| Acetate kinase (AckA) | mpn533 | 0 | 43.8 | Yes | |
| ATP-dependent 6-phosphofructokinase (Pfk) | mpn302 | 0 | 36.0 | Yes | |
| Fructose-bisphosphate aldolase (Fba) | mpn025 | 1 | 31.1 | Yes | |
| Enolase (Eno) | mpn606 | 1 | 49.2 | Yes | Not surface displayed, binds human Plg (25) |
| Glucose-6-phosphate isomerase (Pgi) | mpn250 | 2 | 48.8 | No | Internal helix* |
| Glyceraldehyde-3-phosphate dehydrogenase (GapA) | mpn430 | 1 | 36.8 | Yes | Surface displayed, binds human fibrinogen (24) |
| Lactate dehydrogenase (Ldh) | mpn674 | 0 | 33.9 | Yes | Internal helix* |
| Phosphate acetyltransferase (Pta) | mpn428 | 1 | 35.2 | Yes | |
| Phosphofructokinase (FruK) | mpn079 | 1 | 33.6 | No | |
| Phosphoglycerate kinase (Pgk) | mpn429 | 2 | 44.2 | Yes | |
| Phosphoglycerate mutase (Pgm) | mpn628 | 0 | 56.4 | Yes | |
| Phosphomannomutase (ManB) | mpn066 | 3 | 62.7 | Yes | |
| Pyruvate dehydrogenase complex component E1 subunit alpha (PdhA) | mpn393 | 4 | 40.6 | Yes | Surface displayed, binds human Plg (26) |
| Pyruvate dehydrogenase complex component E1 subunit beta (PdhB) | mpn392 | 1 | 35.9 | Yes | Surface displayed, binds human Plg and fibronectin (25–27) |
| Pyruvate dehydrogenase complex component E2 (PdhC) | mpn391 | 0 | 42.4 | Yes | Surface displayed, binds human Plg (26) |
| Pyruvate dehydrogenase complex component E3 (PdhD) | mpn390 | 1 | 49.4 | Yes | Membrane associated but not surface displayed, binds human Plg (26) |
| Pyruvate kinase (Pyk) | mpn303 | 3 | 57.3 | Yes | |
| Transketolase (Tkt) | mpn082 | 3 | 72.4 | Yes | |
| Triose-phosphate isomerase (TpiA) | mpn629 | 2 | 27.0 | Yes |
a
M. pneumoniae strain M129 (NCBI RefSeq no. NC_000912.1) (34).
b
Schmidl et al. (33).
c
*, http://www.psort.org (PSORTb 3.0.2 [35]).