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Phylogenetic relationship of the pathogenicity locus (PaLoc) of NAP1V and NAP1 strains to that of reference strains (VPI 10463, CD_630, NAP7/RT078_M120, NAP9/RT017_M68, and NAP1/RT027_R20291) and clinical isolates (NAP4, NAP6, and NAP9). The PaLoc sequence of NAP1V clustered together with those of clinical (NAP1-001_5768) and reference NAP1/027 (R20291) strains rather than with sequences from TcdA− TcdB+ variant strains (NAP9/017).
