FIG 7.
Toxinotyping of NAP1 strains. The polymorphisms obtained from the B1 and A3 regions of the tcdA and tcdB genes were analyzed by digestion with AccI (A) and HindIII (H) restriction enzymes. (A) Representation of the amplified regions. (B) The restriction polymorphisms of the tcdB fragments from the NAP1V (toxinotype XXII) and the tcdA-negative tcdB+ NAP9 (toxinotype VIII) strains are indistinguishable and different from the corresponding pattern from the NAP1 strain. (C) The NAP1V and NAP1 (toxinotype III) strains have the same restriction pattern of the tcdA fragment.