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The capacity of the 4496 strain and the 4496ΔΦ and 4496ΔPPI1 mutants to adhere to A549 and Detroit 562 cell monolayers was examined as described in Materials and Methods. Data are means ± standard errors of the means (SEM) of CFU/well for quadruplicate assays. Significant differences in adherence relative to that for the respective 4496 control are indicated as follows: **, P < 0.01; ***, P < 0.001 (Student's t test, two-tailed).
