FIG 5.
MaR1 partially restores LAP neutrophil-impaired phagocytosis of P. gingivalis and A. actinomycetemcomitans. (A) Isolated PMNs (105 cells/well) from LAP patients and HC were incubated (37°C) with MaR1 (0.1 pM to 100 nM) or vehicle (PBS+/+; pH 7.4) alone for 15 min prior to the addition of fluorescence-labeled bacteria (PMN-to-bacterium ratio, 1:50) for 60 min. Phagocytosis was determined using a fluorescence plate reader. Results are expressed as percent changes normalized to levels for HC; results are means ± SEM from n = 5 to 6 donors. P < 0.05 (#), P < 0.01 (##), and P < 0.001 (###) for HC plus MaR1 versus HC plus vehicle; P < 0.05 (*) and P < 0.01 (**) for LAP plus MaR1 versus LAP plus vehicle; P < 0.05 (§), P < 0.01 (§§), and P < 0.001 (§§§) for LAP versus HC plus vehicle. (B) Representative TEM images of LAP neutrophils with P. gingivalis. (Top) Phagocytosis of P. gingivalis. (Bottom) P. gingivalis within the phagolysosome.