FIG 5.

vlsE transcript levels in ptsG mutant and ptsG complemented (ptsGC) strains relative to those in the parental 5A18NP1 B. burgdorferi strain, as determined using qRT-PCR. The results obtained with two biological replicates for each strain from a representative experiment are shown. The Student t test was not significant (P > 0.05) when the data for the WT versus the ptsG mutant and the WT versus the complemented strain were compared. Transcript levels for the enolase gene (BB_0337) of B. burgdorferi B31 were used as an endogenous control to normalize vlsE expression.