TABLE 6.

Validation of differential gene expression of selected genes in the ptsG mutant using qRT-PCR

Gene no.	Gene name	Fold difference (ptsG/WT) determined by:
		RNA-seq	qRT-PCRa
BB_F0041	vlsE	17.3	4.4
BB_0844	Hypothetical gene	13.4	18.6
BB_A24	dbpA	4.4	6.1
BB_B25	dbpB	5.0	1.8
BB_B19	ospC	4.0	1.1
BB_B04	chbC	−2.2	1.5
BB_0408	fruA1	1.3	5.3
BB_0407	manA	1.3	−1.04
BB_A74	osm28	2.2	1.9
BB_0733	plzA	1.6	1.7
BB_0771	rpoS	2.1	1.7
BB_0450	rpoN	1.2	−1.5
BB_0448	hpr	1.2	−1.6

^aResults for the fold difference determined by qRT-PCR analysis are representative of one experiment performed on 3 or 4 technical replicates of the same RNA samples used for RNA-seq.