Fig. 3.

3D-SIM imaging of a FITC-podocin stained normal glomerulus. Maximum intensity projection of a SIM z-stack (79 planes, 9.36μm thick) at 100x. (a) Same region as shown in Fig. 2(b). Squares with (L) denote the capillary lumens. Image shows cross sectional and en face views of podocytes foot processes as they curve around capillary walls. Arrows point to well-resolved podocyte outlines. (b) and (d) Enlargements of two areas marked with arrows in (a). FP = foot process, P = pedicel. (c) and (e) EM sections with similar en face views of podocytes at the same magnification as (b) and (d). The data set can be accessed at [15].