Fig. 4.

Epi-fluorescence imaging of a FITC-podocin stained glomerulus from a patient with nephrotic syndrome (minimal change disease). (a) Section of a whole glomerulus at 20x magnification showing the outlines of many interconnected capillaries, identical in appearance to those in the normal glomerulus in Fig. 2(a). (b) High magnification (100x) image of the region outlined by the white square in (a), showing a cross section (X) and an en face view (E) of one capillary curving through the section. Square with (L) denotes the capillary lumen. The data set can be accessed at [15].