TABLE 4.
Single testing | Simultaneous testing | Sequential two-stage testing | ||||||||
nNO | HSVMA | TEM | nNO + HSVMA | HSVMA + TEM | nNO + HSVMA + TEM |
1. nNO# 2. HSVMA¶ |
1. nNO# 2. TEM¶ |
1. nNO + HSVMA# 2. TEM+ |
1. HSVMA# 2. TEM§ |
|
Subjects | 180 | 180 | 180 | 180 | 180 | 180 | 36 | 36 | 51 | 43 |
Positive | 31 | 31 | 31 | 31 | 31 | 31 | 28 | 28 | 31 | 31 |
Negative | 149 | 149 | 149 | 149 | 149 | 149 | 8 | 8 | 20 | 12 |
True positive | 28 | 31 | 20 | 31 | 31 | 31 | 28 | 19 | 20 | 20 |
True negative | 141 | 137 | 149 | 129 | 137 | 129 | 8 | 8 | 20 | 12 |
False positive | 8 | 12 | 0 | 20 | 12 | 20 | 0 | 0 | 0 | 0 |
False negative | 3 | 0 | 11 | 0 | 0 | 0 | 0 | 9 | 11 | 11 |
Sensitivity (95% CI) | 0.90 (0.74–0.98) | 1.00 (0.89–1.00) | 0.65 (0.45–0.81) | |||||||
Specificity (95% CI) | 0.95 (0.90–0.98) | 0.92 (0.86–0.96) | 1.00 (0.98–1.00) | |||||||
PPV (95% CI) | 0.78 (0.61–0.90) | 0.72 (0.56–0.85) | 1.00 (0.83–1.00) | |||||||
NPV (95% CI) | 0.98 (0.94–1.00) | 1.00 (0.97–1.00) | 0.93 (0.88–0.97) | |||||||
Net sensitivityƒ | 1.00 | 1.00 | 1.00 | 0.90 | 0.61 | 0.65 | 0.65 | |||
Net specificityƒ | 0.87 | 0.92 | 0.87 | 1.00 | 1.00 | 1.00 | 1.00 |
Data are presented as n, unless otherwise stated. PPV: positive predictive value; NPV: negative predictive value. #: n=180; ¶: n=36; +: n=51; §: n=43; ƒ: the net sensitivity and specificity were calculated for combined tests.