. 2015 Dec 3;47(3):837–848. doi: 10.1183/13993003.00749-2015

TABLE 4.

Sensitivity and specificity of high-speed video microscopy analysis (HSVMA), ciliary beat pattern, nasal nitric oxide (nNO) and transmission electron microscopy (TEM) applied as single or combined tests, using simultaneous or sequential testing

	Single testing			Simultaneous testing			Sequential two-stage testing
	nNO	HSVMA	TEM	nNO + HSVMA	HSVMA + TEM	nNO + HSVMA + TEM	1. nNO^# 2. HSVMA^¶	1. nNO^# 2. TEM^¶	1. nNO + HSVMA^# 2. TEM⁺	1. HSVMA^# 2. TEM^§
Subjects	180	180	180	180	180	180	36	36	51	43
Positive	31	31	31	31	31	31	28	28	31	31
Negative	149	149	149	149	149	149	8	8	20	12
True positive	28	31	20	31	31	31	28	19	20	20
True negative	141	137	149	129	137	129	8	8	20	12
False positive	8	12	0	20	12	20	0	0	0	0
False negative	3	0	11	0	0	0	0	9	11	11
Sensitivity (95% CI)	0.90 (0.74–0.98)	1.00 (0.89–1.00)	0.65 (0.45–0.81)
Specificity (95% CI)	0.95 (0.90–0.98)	0.92 (0.86–0.96)	1.00 (0.98–1.00)
PPV (95% CI)	0.78 (0.61–0.90)	0.72 (0.56–0.85)	1.00 (0.83–1.00)
NPV (95% CI)	0.98 (0.94–1.00)	1.00 (0.97–1.00)	0.93 (0.88–0.97)
Net sensitivity^ƒ				1.00	1.00	1.00	0.90	0.61	0.65	0.65
Net specificity^ƒ				0.87	0.92	0.87	1.00	1.00	1.00	1.00

Data are presented as n, unless otherwise stated. PPV: positive predictive value; NPV: negative predictive value. ^#: n=180; ^¶: n=36; ⁺: n=51; ^§: n=43; ^ƒ: the net sensitivity and specificity were calculated for combined tests.