Fig 2. The miscibility limit, βϵ*, of a chaperone–client mixture depends on the chaperone–client binding strength, βϵ_c-s, and the chaperone stoichiometric fraction, x_c.

The chaperone–chaperone interactions have only a minor effect on βϵ*: the miscibility limits of solutions with promiscuous chaperone interfaces, for which βϵ_c-c = βϵ_c-s, are indicated by open circles, while the miscibility limits of solutions in which chaperone–chaperone interactions are prevented are indicated by closed circles. The client–client interaction strength, βϵ_s-s, is sufficient to drive the aggregation of clients in the absence of chaperones.