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. 2016 Feb 29;10(2):e0004485. doi: 10.1371/journal.pntd.0004485

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© 2016 Zampieri et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — Representative melting profiles of hsp70 amplicon 2. Panels (A) and (B) show the melting profiles of American L. (Viannia) species with data organized in normalized melting curves and normalized difference curves, respectively. (Lb): L. (V.) braziliensis; (Ln): L. (V.) naiffi; (Ll): L. (V.) lainsoni; (Lg): L. (V.) guyanensis and (Ls): L. (V.) shawi. Panel (C) shows the amplification curves in relation to the Ct, using DNA of the same species as A and B plus (Lt.): L. (L.) tropica; (Ld): L. (L.) donovani; (Li): L. (L.) infantum; (La): L. (L.) amazonensis; (Lx): L. (L.) mexicana and (Lj): L. (L.) major. Each sample was tested in duplicate.