Table 1. Tm values obtained in the HRM analysis targeting the hsp70 gene of different Leishmania species.

Fifty ng of genomic DNA from each species was tested in duplicate in three independent experiments.

Leishmania species	Tm (°C) Amplicon 1	Tm (°C) Amplicon 2
L. (L.) tropica	84.40 ± 0.02	-
L. (L.) donovani	85.01 ± 0.04	-
L. (L.) infantum chagasi	85.04 ± 0.03	-
L. (L.) major	85.23 ± 0.03	-
L. (L.) amazonensis	85.39 ± 0.03	-
L. (L.) mexicana	85.46 ± 0.06	-
L. (V.) lainsoni	85.60 ± 0.01	84.07 ± 0.01
L. (V.) braziliensis	85.86 ± 0.01	84.51 ± 0.03
L. (V.) guyanensis	86.14 ± 0.02	84.01 ± 0.00
L. (V.) naiffi	86.58 ± 0.11	84.48 ± 0.01
L. (V.) shawi	86.80 ± 0.01	84.07 ± 0.01