Table 3. Call efficiency obtained in the HRM analysis targeting the hsp70 gene of different Leishmania strains.
The “call” column shows the names given by the software to the unknown samples based on names given to the reference samples: L. (L.) infantum chagasi (CHA), L. (L.) amazonensis (AMA), L. (V.) lainsoni (LAI), L. (V.) braziliensis (BRA), L. (V.) guyanensis (GUY), L. (V.) naiffi (NAI) and L. (V.) shawi (SHA). Each condition was tested in duplicate in three independent experiments.
| Leishmania strain / ID | Call | Efficiency (%) |
|---|---|---|
| L. (L.) infantum chagasi / MHOM/BR/74/M2682 | CHA | 67.88 |
| L. (L.) amazonensis / MPHI/BR/99/M12275 | AMA | 83.35 |
| L. (V.) lainsoni / IUBI/BR/91/M13469 | LAI | 73.72 |
| L. (V.) braziliensis / MHOM/BR/2001/M19675 | BRA | 85.47 |
| L. (V.) braziliensis / MHOM/BR/96/M15923 | BRA | 81.12 |
| L. (V.) guyanensis / MHOM/BR/2001/M19869 | GUY | 78.73 |
| L. (V.) naiffi / MDAS/BR/82/M6934 | NAI | 87.75 |
| L. (V.) shawi / MHOM/BR/2001/M19670 | SHA | 91.10 |
| L. (V.) shawi / MHOM/BR/2001/M19664 | SHA | 87.90 |
| L. (V.) shawi / MHOM/BR/1990/M19703 | SHA | 88.10 |