Table 4. Identification of Leishmania in clinical and experimentally infected and field samples by the HRM analysis targeting hsp70 gene.

The hsp70 amplicons 1 or 2 of DNA from each sample were submitted to the HRM analysis. The results were compared with a previous identification performed by SSU rDNA sequencing [17] or g6pd PCR [18]. ^(a) fresh tissue from hamster inoculated with infected sample; ^(b) cell culture of the human isolated strain; ^(c) human fresh tissue; ^(d) human paraffin-embedded tissue; ^(e) experimentally infected BALB/c mice; ^(f) naturally infected Lutzomyia (Lutzomyia) longipalpis; and ^(g) naturally infected Lu. (Nyssomyia) whitmani; (N/A): not applicable.

Sample Source	HRM Identification		Previous Diagnosis	Method
	Amplicon 1	Amplicon 2
Humana	L. (V.) braziliensis	L. (V.) braziliensis	L. (Viannia) sp.	SSU + seq
Humana	L. (V.) braziliensis	L. (V.) braziliensis	L. (Viannia) sp.	SSU + seq
Humana	L. (V.) braziliensis	L. (V.) braziliensis	L. (Viannia) sp.	SSU + seq
Humana	L. (V.) braziliensis	L. (V.) braziliensis	L. (V.) braziliensis	g6pd PCR
Humanb	L. (V.) braziliensis	L. (V.) braziliensis	L. (V.) braziliensis	g6pd PCR
Humanb	L. (V.) braziliensis	L. (V.) braziliensis	L. (V.) braziliensis	g6pd PCR
Humanb	L. (V.) braziliensis	L. (V.) braziliensis	L. (V.) braziliensis	g6pd PCR
Humanc	L. (V.) braziliensis	L. (V.) braziliensis	L. (V.) braziliensis	g6pd PCR
Humand	L. (V.) braziliensis	L. (V.) braziliensis	L. (Viannia) sp.	SSU + seq
Caninea	L. (V.) braziliensis	L. (V.) braziliensis	L. (Viannia) sp.	SSU + seq
Caninea	L. (L.) infantum chagasi	N/A	L. (L.) infantum chagasi	SSU + seq
Mousee	L. (L.) amazonensis	N/A	L. (L.) amazonensis	SSU + seq
Mousee	L. (L.) amazonensis	N/A	L. (L.) amazonensis	SSU + seq
Mousee	L. (V.) braziliensis	L. (V.) braziliensis	L. (V.) braziliensis	g6pd PCR
Phlebotominef	L. (L.) infantum chagasi	N/A	L. (L.) infantum chagasi	SSU + seq
Phlebotomineg	L. (V.) braziliensis	L. (V.) braziliensis	L. (V.) braziliensis	g6pd PCR