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. 2016 Mar 1;6:22. doi: 10.3389/fcimb.2016.00022

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Copyright © 2016 Truchan, Cockburn, Hebert, Magunda, Noh and Carlyon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The AmV interacts with the ER as visualized by TEM. A. marginale infected ISE6 cells were examined by TEM. White hatched boxes denote areas in the images presented in (A,C) that are presented as enlarged panels in (B,D), respectively. The white arrow in (A) denotes a RER-AmV contact site. White arrowheads in (B,D) point to ribosomes that label the cytosolic face of the AmV membrane. Thin black arrows in (A–C) demarcate vesicles within the AmV lumen in close apposition to A. marginale organisms. Scale bars, 0.5 μm. Results shown are representative of two experiments in which a combined total of over 40 different electron micrographs were analyzed.