Figure 11.

ApV association with the ER is Rab10-independent. HEK-293T cells were treated with Rab10-targeting or non-targeting siRNA for 72 h. (A) Lysates of non-targeting or Rab10 siRNA treated cells were examined by Western blot for Rab10 knockdown. (B) Following siRNA treatment, the cells were infected with A. phagocytophilum for 48 h, fixed, screened with antibodies against APH0032 and calreticulin or derlin-1, and examined using LSCM. Host cell nuclei and bacterial DNA were stained with DAPI. Regions demarcated by hatched line boxes are magnified in the corresponding inset images that are denoted by a solid line boxes. Arrows point to regions of expansive cisternae, which is characteristic ER morphology in Rab10-depleted cells. Scale bars, 5 μm. Results shown are representative of two experiments with similar results.