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. 2016 Mar 1;6:22. doi: 10.3389/fcimb.2016.00022

Figure 3.

Figure 3

Derlin-1 immunolabeling of intravacuolar A. phagocytophilum bacteria is specific and is reproducible among different derlin-1 antibodies. (A,B) Derlin-1 antibody does not cross-react with any A. phagocytophilum protein. Western blotted lysates of uninfected HL-60 cells and host cell-free A. phagocytophilum (Ap) bacteria (A) or A. phagocytophilum infected derlin-1 siRNA treated (Der1), non-targeting siRNA treated (NT), or uninfected, untreated (U) control HEK-293T cells (B) were probed with antibodies against derlin-1, A. phagocytophilum P44, or ß-actin. (C) Two different derlin-1 antibodies produce comparable immunolabeling patterns of the host cell ER and intravacuolar A. phagocytophilum organisms. A. phagocytophilum and/or uninfected RF/6A cells were screened with derlin-1 antibodies obtained from two different commercial sources. Scale bars, 5 μm. Results shown are representative of two experiments with similar results.