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. 2016 Mar 1;6:22. doi: 10.3389/fcimb.2016.00022

Figure 4.

Figure 4

Ectopically overexpressed mCherry-derlin-1 is delivered into and inhibits development of the ApV, and knocking down derlin-1 increases the A. phagocytophilum bacterial load. (A) mCherry-derlin-1 is delivered into the ApV, associated with intravacuolar A. phagocytophilum bacteria, and inhibits ApV development. HEK-293T cells transfected to express mCherry, mCherry-derlin-1, or mock-transfected were incubated with A. phagocytophilum. At 24 h, the cells were fixed, stained with DAPI, and visualized using LSCM. The white arrow in (A) denotes a small mCherry-derlin-1-positive ApV. Scale bars, 0.5 μm. (B) The A. phagocytophilum load is increased in derlin-1 siRNA-treated cells. HEK-293T cells were treated with derlin-1-targeting or non-targeting (NT) siRNA for 72 h. Following siRNA treatment, the cells were infected with A. phagocytophilum for 24 h and total DNA was isolated and subjected to QPCR analysis.