Figure 7.

SIM analyses confirm that calreticulin- and derlin-1-positive vesicles are present in the ApV and AmV lumen in close proximity to intravacuolar Anaplasma spp. bacteria. A. phagocytophilum (A) and A. marginale infected RF/6A cells (B) that had been screened with antibodies against calreticulin or derlin-1 were examined by SIM. The regions in the Merge panels that are demarcated by hatched line boxes indicate the regions that are magnified in the Enlargement panels. Host cell nuclei and bacterial DNA were stained with DAPI (blue). Scale bars, 5 μm. Results shown are representative of two experiments with similar results.